Hyperglycaemia impairs pAMPK
, but not pAMPK
activation. (A-C) BxPC-3 and (D-F) MIAPaCa-2 cells were treated with or without metformin in normal (5 mM) or high (25 mM) glucose-containing media with 1% FBS for 24 h and processed for immunoblot analysis using antibodies against pAMPKThr172, pAMPKSer485 and AMPK. GAPDH is shown as a loading control. Graphs display densitometry measurements of relative levels of AMPK protein phosphorylation at 5 mM (black bars) or 25 mM (striped bars) glucose conditions and represent means ± SE of three independent experiments. P-values are based on two-way ANOVA with Bonferroni post hoc test. N.S. not significant. One representative blot from three independent experiments is shown.