Figure 3

Chronic and complete loss of all Vegf gene products augments resistance to hypoxia-induced apoptosis. (A,B) Parental (VEGF^+/+) or VEGF-KO (VEGF^-/-) cell lines were exposed to normoxia for 2 days or hypoxia for 3 days in the absence (none) or presence of a VEGFR-TKI, then apoptotic cells were measured by TUNEL assay. *P < 0.01., compared with the respective normoxic control cells in each cell lines (n=6, means ± S.D.). (C,D) Expression levels of Plgf (C) and Vegf-b (D) were measured by quantitative RT-PCR (n=4, means ± S.D.). *P < 0.01., compared with parental (VEGF^+/+) cells.