Figure 3

RAB34 is the direct target of miR-9. A, Western blot analysis of RAB34 in ST30 cells transfected with miR-9 mimic or negative control. GAPDH was used as house-keeping gene. B, Dual luciferase reporter assay. 293T cells were transfected with RAB34-wild type 3′UTR vectors or mutant 3′UTR vectors together with miR-9 mimic or its negative control. Luciferase activity was measured 48 h after co-transfection, and the error bars represent SD. A decrease of the luciferase activity was observed in miR-9 over-expressing cells (* P= 0.000).