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Table 4 Analytical sensitivity of plasmid DNA blends

From: Analytic performance studies and clinical reproducibility of a real-time PCRassay for the detection of epidermal growth factor receptor gene mutations informalin-fixed paraffin-embedded tissue specimens of non-small cell lungcancer

EGFR Mutation

Nucleic Acid Sequence

Amount of DNA in 5% copy equivalent (ng/25uL) to achieve≥95% “Mutation Detected” Rate(N = 72 replicates/plasmid)

Exon 18 G719A

2156 G > C

3.13

Exon 19 Deletion

2235-2249del15

0.78

Exon 20 S768I

2303 G > T

0.78

Exon 20 T790M

2369 C > T

3.13

Exon 20 Insertion

2307_2308ins9 GCCAGCGTG

3.13

Exon 21 L858R

2573 T > G

0.78