Analytic performance studies and clinical reproducibility of a real-time PCRassay for the detection of epidermal growth factor receptor gene mutations informalin-fixed paraffin-embedded tissue specimens of non-small cell lungcancer

Table 3 Analytical sensitivity of formalin-fixed paraffin-embedded tissue DNAblends

EGFR mutation	Mutant specimen No.	EGFR nucleic acid sequence	Lowest % mutation in the 50 ng/PCR well input to achieve≥95% “mutation detected” rate(N = 24 replicates)
Exon 19 deletion	1	2235_2249del15	1.39
	2	2236_2250del15	2.53
	3	2238_2252del15	2.37
L858R	4	2573 T > G	3.96
	5	2573 T > G	4.19
	6	2573 T > G	4.33
	7	2573 T > G	5.32
T790M	7	2369 C > T	2.04
S768I	8	2303 G > T	2.42
Exon 20 insertion	9	2310_2311insGGT	1.26
G719X	10	2156 G > C	2.46
G719X	8	2155 G > T	5.56

ISSN: 1471-2407