Serum HMGB1 levels in patients with MPM and non-MPM subjects. (A) Sensitivity and specificity of serum HMGB1 for distinguishing patients with MPM from non-MPM subjects (ROC curve). An analysis that included 61 MPM patients and 45 non-MPM subjects with a history of asbestos exposure revealed an AUC of 0.674 (95% CI: 0.589-0.758). At a cut-off value of 9.0 ng/ml, diagnostic sensitivity was 34.4% and specificity was 100%. (B) Serum HMGB1 levels in non-MPM subjects and MPM patients were measured as described in the Methods.