Evaluation of HMGB1 production in mesothelioma and mesothelial cells. H28, H2052, H2452, MSTO-211H mesothelioma cell lines and human mesothelial cell line MeT-5A were cultured for 24 hours in serum-free medium. The concentration of HMGB1 in the culture supernatant of all cells was measured as described in the Methods. Results are indicated as the mean ± SD of three separate experiments in triplicate. The Bonferroni/Dunn multiple comparisons test was used.