Treatment with bethanechol increases AOM-induced murine colon tumor number and volume. A. Representative dissecting microscopic images of AOM-induced colon tumors from mice that were fed water (control; N = 8) or water containing bethanechol (N = 7). B. Representative H&E-stained sections (magnification, 200×). Size bars represent 100 microns. a. Representative scanned image of entire distal half of colon that contains tumors. b. Arrow indicates an adenoma. c. Arrowhead indicates an adenocarcinoma. C. Effect of bethanechol treatment on tumor number. D. Effect of bethanechol treatment on tumor volume. Bars in C and D represents mean ± SE.