Figure 2

CRHBP protein expression and tissue localization in cc-RCC and normal kidney. A) Western blotting shows a tumor specific loss of CRHBP protein in lane 2 and 4. Positive signals for CRHBP were observed in most of the normal specimens. Detection of β-Tubulin has been used as an internal loading control. B) CRHBP immuno-signalling in proximal tubuli (green arrows), glomeruli (yellow arrows) and lack of immunopositivity in distal tubules of normal kidney (red arrows). C) Immunopositivity for nephrin detected in glomeruli and proximal tubules in normal kidney. D) Double immunofluorescence staining of nephrin (red) and CRHBP (green) demonstrating co-expression in glomeruli and proximal tubules of normal renal tissues. E) Double immunofluorescence staining of CRHBP (green) and distal marker protein MUC-1 (red) in normal kidney F) Invasion front tissue demonstrating reduction of CRHBP protein immunosignaling when normal (left side) and tumor tissue areas (right side) are compared G) CRHBP immunofluorescence staining (green) with nuclear counterstaining using Dapi (blue) in tumor tissue of cc-RCC.