Effect of P61A6 on tumorigenicity of H358 cells in vivo and intracellular distribution of RhoA in H358 xenografts. The treatment was started 3 weeks after subcutaneous inoculation of the cells when palpable tumors reached 3–5 mm in diameter. 5 times/week i.p. injections were performed until the end of experiment. A. Animals injected with P61A6 (right) exhibited smaller tumor compared to controls in situ (left). B. Comparison of the eight largest extirpated tumors from control animals (top) and the eight largest from animals treated with P61A6 (bottom) demonstrates the effects of treatment on xenograft tumor growth. C. The average tumor volumes of P61A6-treated and untreated groups is shown. D. Protein extracted from cytosolic and membrane fractions of tumors from P61A6-treated and untreated animals was western blotted for RhoA to evaluate the RhoA association with membrane. (M, membrane fraction; C, cytosolic fraction).