Effects of P61A6 on cell cycle progression. A. H358 cells were treated with DMSO or P61A6 for 48 hours. Cell cycle profiles were monitored by flow cytometry. The percentages of cells in each phase of the cell cycle are indicated by different shades. Shown are representative results from three independent experiments. B. Whole cell lysates from cells treated with DMSO or P61A6 for 48 hours were prepared and resolved on SDS-PAGE for immunoblotting using antibodies against cyclin D1/2, p21CIP1/WAF1, p27Kip1, or RhoGDI (loading control). Data shown are representative of two independent experiments.