Skip to main content
Figure 3 | BMC Cancer

Figure 3

From: Expressional alterations in functional ultra-conserved non-coding rnas in response to all-transretinoic acid - induced differentiation in neuroblastoma cells

Figure 3

Functional analysis of T-UCRs. T-UC.324 and T-UC.300A transcripts were knocked down using custom designed siRNA (Dharmacon). (A) Knockdown of T-UC.324 significantly abrogated its up-regulation following ATRA-treatment, (B) but had no effect on cell viability. (C) A significant decrease in T-UC.300A levels was observed following siRNA transfection. (D) Knockdown of T-UC.300A resulted in a decrease in cell invasion as shown by MATRIGEL invasion assay (p<0.01) and (E) cell viability as shown by acid phosphatase assay (P<0.01). (F) Reduction in cell viability following T-UC.300A knockdown was shown by BrdU assay to be due to a decrease in cell proliferation (p<0.05). (G) Knockdown of neither T-UC.324 nor T-UC.300A had any effect on apoptosis.

Back to article page