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Figure 5 | BMC Cancer

Figure 5

From: JNK signaling maintains the mesenchymal properties of multi-drug resistant human epidermoid carcinoma KB cells through snail and twist1

Figure 5

Snail and twist1 are both involved in maintaining the mesenchymal properties of KB/VCR cells controlled by JNK signaling. A. RT-PCR analysis for the expression of transcriptional factors associated with EMT in KB and KB/VCR cells. Total RNA of KB and KB/VCR cells was extracted and reversed to cDNA, which was subsequently subjected to PCR analysis with specific primers as indicated in materials and methods. B. Expression of snail and twist1 at mRNA level was determined by real time PCR analysis (*P < 0.01; **P < 0.05). C. Expression of snail and twist1 at protein level was determined by western blot analysis. D-E. Interfering with JNK1/2 with SP (D) or JNK1/2 shRNA (E) suppressed the expression of snail and twist1 in KB/VCR cells. KB cells or KB/VCR cells treated with SP 10 μM after 24 h or after infected with JNK1/2 shRNA were lysed and subjected to western blot analysis using specific antibodies. Western blot for β-actin was used as a loading control. F. Knockdown of c-Jun with siRNA reduces the expression of snail and twist1 in KB/VCR cells. KB cells or KB/VCR cells transfected with c-Jun siRNA or control were lysed, and used for western blot analysis with specific antibodies. In all cases, blots are representative of three to four separate experiments.

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