Figure 3
Knockdown of JNK1/2 reverses the mesenchymal phenotypes of KB/VCR cells. A. Knockdown of JNK1/2 by lentiviruses carrying JNK1/2 shRNA. KB/VCR infected with lentiviruses carrying shRNA control or JNK1/2 shRNA were lysed after infection, and used for Western blot analysis. Western blot for GAPDH was used as a loading control. Data are similar results from three independent experiments. B. Knockdown the expression of JNK1/2 by JNK1/2 shRNA results in KB/VCR cells acquiring of epithelial cobblestone like morphology contrast to that of shRNA control. (×10). C. Knockdown of the expression of JNK1/2 significantly increases the expression of E-cadherin and obviously decreases the expression of N-cadherin, vimentin in KB/VCR cells. The expression of E-cadherin, N-cadherin and vimentin was examined as described above in KB/VCR cells after infection with lentiviruses carrying shRNA control or JNK1/2 shRNA. Data are representative blots from three independent experiments. D-E. Knockdown of the expression of JNK1/2 significantly inhibits the migration (C: *P < 0.001 compared to KB; **P < 0.01 compared to control shRNA) and invasion (D: *P < 0.05 compared to KB; **P < 0.05 compared to control shRNA) of KB/VCR cells in response to serum. The migration and invasion of KB/VCR cells in response to serum were examined as described above after infection with lentiviruses carrying shRNA control or JNK1/2 shRNA. Data are expressed as average ± SD of results obtained from three separated experiments.