Figure 8From: Characterization of in vivo chemoresistant human hepatocellular carcinoma cells with transendothelial differentiation capacities Anchorage independent growth. Multicellular spheroids grew from a single cell suspension of (A) parental HUH-7 cells (HUH-wt), (B) in vivo passaged HUH7 cells (HUH-PAS) and (C) chemoresistant cells (HUH-REISO) in low melting agarose for 42 days and pictures were taken under a phase contrast microscop. Spheroid tissue organization was detected by H/E staining of cryoslides. (D) Parental HUH-7 cells (HUH-wt), (E) in vivo passaged HUH7 cells (HUH-PAS) and (F) chemoresistant cells (HUH-REISO).Back to article page