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Figure 3 | BMC Cancer

Figure 3

From: Characterization of in vivo chemoresistant human hepatocellular carcinoma cells with transendothelial differentiation capacities

Figure 3

Immunohistochemical analysis for vascular markers in HUH-7 tumors. Cryosections (5 μm) of untreated control tumors (HUH-wt) (A) and CPA treated, chemoresistant tumors HUH-REISO (B) were fixed with 4% PFA and stained with antibodies for murine CD31/PECAM-1 (green) and laminin (yellow). Significant changes in the arrangement of laminin and CD31/PECAM-1 positive endothelial cells were detected in CPA treated tumors versus control tumors. Functional blood flow was visualized by intravenous application of Hoechst 33258 dye (blue). Additional immunohistochemically stained cryosections of untreated HUH-wt (C-F) tumors, and HUH-PAS (G-J) or HUH-REISO (K-N) tumors after two times CPA treatment are shown. Staining for murine CD31/PECAM-1 (D/H/L, green, highlighted with black arrows) and human CD31/PECAM-1 (E/I/M, magenta, highlighted with white arrows) and cell nuclei were counterstained with DAPI (C/G/K). Adjacent to signals from murine CD31/PECAM-1, significant human CD31/PECAM-1 expression was detected in (K-N, HUH-REISO) chemoresistant, CPA treated tumors, whereas human CD31/PECAM-1 expression was not detected in (C-F, HUH-wt and G-J, HUH-PAS) control tumors.

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