Figure 2

Macroscopic appearance, tumor histology and functional blood flow. (A-C) Untreated parental tumor (HUH-wt) and (D-E) CPA treated in vivo resistant tumor at treatment endpoint (HUH-REISO), all collected at day 25 after tumor cell implantation. Cryosections (8 μm) were fixed with 4% paraformaldehyde (PFA) and subjected to H/E staining (B) untreated parental tumor (HUH-wt) and (E) in vivo resistant tumor at treatment endpoint (HUH-REISO). Functional blood flow was visualized by intravenous application of Hoechst 33258 dye (blue). (C) Untreated parental tumor (HUH-wt) and (F) in vivo resistant tumor at treatment endpoint (HUH-REISO).