Skip to main content
Figure 3 | BMC Cancer

Figure 3

From: Membrane Type 1 Matrix Metalloproteinase induces an epithelial to mesenchymal transition and cancer stem cell-like properties in SCC9 cells

Figure 3

MT1-MMP induces SCC9 cells to undergo EMT is associated with an increased level of Twist and ZEB and through repressing the transcription of E-cadherin. (A) Real-time RT-PCR was performed to detect the expression of CDH1 that encoding E-cadherin in SCC9 cells, stable SCC9 cells expressing an empty vector (SCC9-N) and MT1-MMP (SCC9-M). GAPDH was used as a control. Each bar represents the mean ± s.d. ***P<0.001. (B) Real-time RT-PCR was performed to detect the key transcription factors that repressed CDH1 as inducers of EMT, including Snail, Slug, Twist, ZEB1 and ZEB2. GAPDH was used as a control. Each bar represents the mean ± s.d. **P<0.01. (C) Western blot was performed to analyze the expression of transcriptional repression factors on the protein level. β-actin was employed as a loading control. (D and E) Quantitative determination of mRNA expression of transcription factors on the SCC9-M cells treated with TIMP1 and TIMP2. Each bar represents the mean ± s.d. *P<0.05. The western blot analysis was performed to assess the expression of transcription factors on the SCC9-M cells treated with TIMP1 and TIMP2 on the protein level. β-actin was employed as a loading control. (F) The examination of the shedding of E-cadherin extracellular domain in conditioned medium and cell surface of SCC9, SCC9-N and SCC9-M.

Back to article page