Figure 5
From: Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro
Synergistic interaction of temsirolimus with various anti-IGF-1R molecules. Cytotoxicity assays were performed as described in Materials and Methods. A dose/response curve with temsirolimus was made from 1x10-6 to 6.1x10-11 M. To one set of temsirolimus wells was added a constant amount of (A) hR1 or (B) Hex-hR1 at 100 nM, 10 nM, or 1 nM. (C) For 1R-2b, a constant amount of 26, 2.6, or 0.26 nM was added to the wells. After 96 h incubation, growth relative to untreated cells was determined and the amount of temsirolimus required to inhibit cell growth by 50% was calculated for temsirolimus alone or when used in combination with hR1, Hex-hR1, or 1R-2b. C.I. values were determined as described, with a value less than 1 indicative of synergy. Dotted lines indicate growth-inhibition of each anti-IGF-1R agent alone. Data are shown as mean ± standard deviation.