Figure 3

In vitro sensitivity to anti-IGF-1R treatment. (A) Various RCC cell lines were plated in 96-well plates overnight in SFM media before being stimulate with 20 ng/mL of IGF-1. Cells were incubated a further 96 h before cell viability determined as described in Materials and Methods. Growth relative to cells grown only in SFM are shown in the graph. Large dotted line indicates growth of cells in SFM while smaller dotted line indicates point of 50% increase in growth. RH30 served as a positive control cell line. (B) In vitro cytotoxicity assay was run as described in Materials and Methods and compared hR1, Hex-hR1, and positive control MAB391. After 96-h incubation, growth as a percent of untreated cells is shown in the graphs. The table shows maximum growth-inhibition achieved in the various cell lines with each antibody and the concentration required to achieve that inhibition. Data are shown as mean ± standard deviation.