Figure 3

IL-11 neutralizing antibody reduces breast cancer conditioned media’s ability to give rise to osteoclast progenitors. (A) Bone marrow flushes were cultured in α-MEM containing IL-11 (10 ng/ml) with control IgG (IgG) or IL-11 neutralizing antibody (nIL-11, 2ug/ml) for 6 days. Surviving cells were counted at day 3, 4, 5, and 6. (B) Bone marrow flushes were cultured in α-MEM (0%) or α-MEM with increasing concentrations (5%, 10%, 20% or 40%) of MDA-MB-231 conditioned media for 6 days. Surviving cells were counted at day 3, 4, 5, and 6. (C) Bone marrow flushes were cultured in α-MEM (ctl), 20% MDA-MB-231 conditioned α-MEM (CM), 20% MDA-MB-231 conditioned α-MEM with control IgG (CM + IgG, 5 ug/ml), or 20% MDA-MB-231 conditioned α-MEM with IL-11 neutralizing antibody (CM + nIL-11, 5ug/ml). Surviving cells were counted at day 3, 4, 5, and 6. All data were repeated independently three times and are expressed as a mean +/− S.E, *, p < 0.05; **, p < 0.004.