Figure 4
From: Chemoprevention utility of silibinin and Cdk4 pathway inhibition in Apc −/+ ice
Cell growth, viability, and Ki67 staining of the silibinin treated HT-29 cells. A: HT-29 cells were incubated with vehicle (DMSO) and silibinin, at 0, 0.1, 1, 10, and100 μM and cells or events counted at 24 hours. Results shown are the average of triplicate determinations. Results are expressed as the mean and normalized against their own control, vehicle. (Bar = SEM. * P < 0.05 versus control). B: Analysis of proliferation in HT-29 treated cells by flow cytometry.