L1CAM expression in endometrial carcinoma cell lines. (A) mRNA was isolated from the indicated cell lines, transcribed to cDNA and subjected to quantitative RT-PCR analysis. β-actin served as internal standard. (B) FACS analysis of selected cell lines. Cells were stained with mAb L1-9.3 (solid line) to the ectodomain of L1CAM followed by PE-conjugated goat anti mouse IgG. For background control (shaded curves) the primary antibody was omitted.