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Figure 1 | BMC Cancer

Figure 1

From: TRAIL and proteasome inhibitors combination induces a robust apoptosis in human malignant pleural mesothelioma cells through Mcl-1 and Akt protein cleavages

Figure 1

MG132 induces a limited apoptosis in MPM cells. (A) Cell viability assay using WST-1 reagent indicates that 0.5-1 μM MG132 induces a significant cell death at 72 h after treatment in both NCI-H2452 and NCI-H2052 cells, but not in NCI-H28 cells. Values are expressed as the means ± SD of two experiments. *p < .05 versus control. (B) Western blotting demonstrates that 0.5-1 μM MG132 induces protein cleavages for caspase 3 and PARP at 42 h after treatment in NCI-H2452 and NCI-H2052 cells, but not in NCI-H28 cells. The treatments cause a significant Mcl-1 protein elevation in all three MPM cell lines. NCI-H28 and NCI-H2052 cells exhibit a much higher P-Akt protein level than NCI-H2452 cells, whereas the P-STAT3 protein is only detectable in NCI-H2452 and NCI-H2052 cells. Protein cleavage fragments in Western blots are indicated by arrows.

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BMC Cancer

ISSN: 1471-2407

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