Figure 4

Knockdown of WDR66 gene affects cell motility and cell growth. A: Cell motility assays showed that knockdown of WDR66 reduced cell migration after 16 hours. About 35% of the siWDR66 cells were migrated in comparison to that of the mock control cells. The differences between siWDR66 and Allstar (negative control siRNA) cells were significant (p = 0.0032) using the paired t test. The data shown are representatives of three independent experiments and each done in quadruplicate. B: Knockdown of WDR66 leads to suppression of cell growth. A total of 1.5×10⁴ cells were seeded at day 0. WDR66 knockdown cells grew slower than cells treated with Allstar (negative control siRNA). The differences between siWDR66 and Allstar (negative control siRNA) treated cells were highly significant (p = 0. 0098). C: Wound-healing assays show that knockdown of WDR66 reduces cell motility. Representative images are shown. Images are taken immediately after insert was removed and 8 hours later. Original magnification is x400.