Knockdown of WDR66 gene affects expression of vimentin and occludin. A: mRNA expression of the VIM and OCLN gene in the original training cohort determined by microarray analysis. Array data analysis was performed on 18 healthy normal esophageal epithelium (NE) and 10 esophageal squamous cell carcinoma (ESCC) samples. Gene expression is presented as normalized (log2 scale) signal intensity of the genes of interest. VIM and OCLN are significantly differentially expressed in ESCC compared with NE (corrected p-value: VIM p = 0.0008; OCLD p < 0.0001). VIM expression level is low in NE but high in ESCC cases, whereas OCLN expression is high in NE but low in ESCC cases. The horizontal axis depicts the patient groups ESCC and NE. (∗∗∗ P < 0.001) B: Knockdown of WDR66 affects mRNA expression of VIM and OCLN. Gene expression is presented as normalized (log scale) signal intensity for mRNA expression levels of VIM and OCLN gene. Gene expression level of VIM was significantly down regulated whereas OCLN expression was significantly higher in cells treated with WDR66 siRNA in comparison to NTC (KYSE520) and Allstar (negative control siRNA) (corrected p-value: VIM p = 0.0286; OCLD p = 0.0186). Data are representatives of four independent experiments. (∗p < 0.05). C: Detection of vimentin and occludin protein by immunoblotting of KYSE520 cells treated with WDR66 siRNA in comparison to NTC (KYSE520) and Allstar (negative control siRNA). β-actin was used as loading control. Vimentin expression was significantly down regulated while occludin was significantly higher expressed in cells treated with WDR66 siRNA.