High WDR66 mRNA expression is associated with poor survival in ESCC patients. Kaplan-Meier analysis of survival of grouped according to WDR66 expression as measured by quantitative real-time RT-PCR. Grouping of patients according to median of qRT-PCR measurements was done as follows: WDR66 ≤ 125, WDR66 low (n = 12); WDR66 > 125, WDR66 high (n = 13). After choosing an optimal cut-point, analysis for WDR66 was done using maximally selected rank statistics. The group with patients expressing WDR66 at low levels showed a significantly better overall survival compared with the group with high levels of WDR66 expression (P = 0.031; log rank).