Figure 5

Predictive inhibition capacity of Δ40p53 isoform. (A) Mathematical approach to analyze probabilities of the tetramer formation, containing different numbers of TAp53 and Δ40p53 monomers (adapted from [21]). The equations defining the probabilities (P) of forming different tetramers are indicated, where r is the ratio (concentration of Δ40p53/concentration of TAp53). p53 denotes TAp53 and Δ40, Δ40p53. (B) Prediction of TAp53/Δ40p53 hetero-tetramer formation based on β-galactosidase assay results using cells transfected with vectors according to the proportions defined in (A). The predicted inhibition curves (dotted lines) were calculated from the probability of forming the different tetramers shown in (A). Transcriptional activities measured using a β-galactosidase reporter gene as described in Figure 4 were plotted. Green triangles represent the different values obtained in six independent experiments done in triplicate in H1299 cells and green crosses joined by dotted line, the average curve. Blue circles represent the different values obtained in three independent experiments done in Saos-2 cells and blue crosses joined by dotted line, the average curve. P-values are given for the comparison between H1299 and Saos2 cells. *P < 0.05 and ***P < 0.001.