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Figure 3 | BMC Cancer

Figure 3

From: Effects of Δ40p53, an isoform of p53 lacking the N-terminus, on transactivation capacity of the tumor suppressor protein p53

Figure 3

DNA-binding activity of TAp53 and Δ40p53. Electrophoretic mobility shift assay (EMSA) detecting DNA-binding activity. p53-null H1299 cells were co-transfected with a constant amount of pcDNA3-TAp53 plasmid (0.5 μg/ml) in the presence of increasing amounts of pcDNA3-Δ40p53 plasmid (0.5 and 1.5 μg/ml). Nuclear extracts were incubated with a 32P-labeled p53con oligonucleotide, with or without PAb421, which stabilizes and super-shifts p53: DNA complexes. DO-7 induces a super-super-shift of p53/DNA complexes. DNA: p53 shifts, DNA: p53:PAb421 super-shifts, DNA: p53:PAb421:DO-7 super-super-shifts are indicated by black arrows.

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BMC Cancer

ISSN: 1471-2407

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