Figure 2

Formation of hetero-oligomers between TAp53 and Δ40p53. (A) Immunoprecipitation of TAp53 using DO-7 antibody in BALB/c 10.1 cells. Immunoprecipitation was performed with DO-7 antibody (+) or without DO-7 antibody as a negative control (−) using increasing amounts of TAp53 transfected plasmid. Immunoprecipitated proteins were analyzed by immunoblotting using CM1 polyclonal antibody. TAp53 immunoprecipitation is enhanced when the amount of transfected plasmid is increased. pcDNA3: empty vector; input: unprecipitated proteins issued from cell extract; black arrow: TAp53. (B) Immunoprecipitation of TAp53 or Δ40p53 using DO-7 antibody. Immunoprecipitation was performed using either TAp53 or Δ40p53 transfected plasmid. (C) Co-immunoprecipitation of TAp53 and Δ40p53 using different amount of transfected TAp53 plasmid. Same experiment of immunoprecipitation as described in (A) and (B), using co-transfection of both TAp53 (0.5 and 1.5 μg/ml) and Δ40p53 (0.5 μg/ml). In each condition, the two p53 isoforms interact but in particular when they are expressed in the same amount. Black arrow: TAp53; white arrow: Δ40p53.