Figure 1

Pictures of 001-CSS patient melanoma and CAT scans. A- Micrograph of the primary tumor (hematoxylin/eosin staining) showing highly melanotic tumor cells and scarce lymphocytes in the tumor periphery. Original magnification = 200×; scale bar = 20 μm. B- DTH reaction to the vaccine at the right forearm is shown as an example of patient’s immune reaction after receiving eight doses of CSF470 vaccine plus BCG plus GM-CSF, as described in the text. C and D- CAT scan pictures of the abdomen obtained by the end of the clinical study (August 2011). No melanoma lesions were detected. E and F- CAT scan pictures obtained at March 2012, three months after the patient received Pg treatment, showing multiple lesions in the liver, suprarenal glands and spleen. G- Micrograph of a hepatic needle biopsy (hematoxylin/eosin staining) confirming the presence of multiple melanoma metastases in the hepatic parenchyma. Original magnification = 200×; scale bar = 20 μm. H- Immunohistochemical staining for PgR in the primary tumor was negative; I- positive control for PgR staining (breast carcinoma). For both, original magnification = 400×; scale bar = 10 μm.