Figure 4

Influence of αB-crystallin knock-down on cell migration determined with gap closure assay. Knockdown of αB-crystallin mRNA expression in UT-SCC-15 cells by two different siRNAs, αB1 and αB2, compared to negative control siRNA LUC (Luciferase) as measured by quantitative PCR (a). Representative micrographs of the gap closure by UT-SCC-15 cells after 0 and 180 minutes at 10x magnification. (b). Relative gap size between 0 and 180 minutes of UT-SCC-15 cells transfected with the αB1, αB2 or LUC siRNA (c). Statistical analysis was performed using One-way ANOVA and Tukey’s Multiple Comparison Test. *** P < 0.001, * 0.01 < P < 0.05.