Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: The impact of KRAS mutations on VEGF-A production and tumour vascular network

Figure 1

HIF-1α, GLUT-1 and VEGF-A in cultured KRAS ASP13 and CYS12 transfectants. A) HIF-1α protein expression was measured by western blot in NIH3T3 control cells (0), three independent clones KRAS CYS12 mutant (1, 2 and 3 show different transfected clones) and two independent clones KRAS ASP13 mutant (Clones 4 and 5). Tubulin was measured as a loading control. B) Luciferase activity after transfection of HIF-1α-response element promoter in NIH3T3, clone 3 KRAS CYS12 mutant and clone 4 KRAS ASP13 mutant. Results are average ± SE of three independent experiments. Activity in ASP13 mutants was significantly lower than in CYS12 mutants (P < 0.05). C) Protein extracts were obtained from clone 4 KRAS ASP13 mutant and from clone 3 KRAS CYS12 mutant. GLUT-1, VEGF-A and Tubulin (as a loading control) were immunodetected by western blotting. A representative blot is shown. The electrophoretic pattern of GLUT-1 depicts its glycosylation.

Back to article page