Figure 2

LMP1 induced phosphorylation of histone H3 at Ser10 in CNE1 cells. (A) CNE1G and CNE1GL cells were serum-starved for 36 h and subjected to immunocytochemical staining (×200). No expression and positive staining of LMP1 in CNE1G and CNE1GL cells (a, b). Different expression of histone H3 phosphorylation at Ser10 in CNE1G and CNE1GL cells (c, d). (B) CNE1 cells were transfected with different amount of pcDNA3.0 (1.25-2.5 μg) or pcDNA3.0-LMP1 (1.25-2.5 μg). After 24h of transfection, cells were starved for another 24 h. Total protein and histone protein were extracted and the expressions of LMP1 and phosphorylated histone H3 were detected by Western blot analysis. β-actin and total histone H3 were used as loading controls.