Figure 3
Effect of 1,25(OH) 2 D 3 on expression of CYP24A1, CA2, CD14, DPP4, SHE, BMP6 and ILRL1 in breast cancer slices. Tumor specimens from 12–16 patients were treated or not (control) with 1,25(OH)2D3 (0.5nM or 100nM) for 24 hours and analyzed by qPCR. Relative expression was calculated as 2-ΔΔCT, using GAPDH as internal control and the average value of the target gene in control samples, as reference. Horizontal line represents the median value of gene expression. Friedman test, p value inside the box; * (over the horizontal bracket): p ≤ 0.05, Wilcoxon signed ranks test.