Figure 5
HIF-1α promotes transcription of SNAI1 under hypoxia condition. (A) Schematic diagram of SNAI1 promoter-luciferase construct was shown with the location of HRE. (HRE: hypoxia response element) (B) SMMC-7721 cells were transfected with pGL3-basic vector or a series of pGL3 vectors containing truncated SNAI1 promoters or promoters with mutated HRE (P1, P2, P3, P4, M1, M2 and MM) along with renilla luciferase expression vector. Luciferase assay was performed after 2 days. The firefly luciferase activity was normalized by renilla luciferase activity. Data were shown as mean ± SD of three independent experiments. (C) A model was shown for effect of HIF-1α on SNAI1-mediated EMT in HCC.