Figure 6
Overexpression of PRAF3 suppressed the expression and activity of MMP-9 and affects the membranous location of CCR5. 1.0 × 106 Eca109 and TE-1 cells were infected with Ad.PRAF3 (or Ad.Null) at a MOI of 100. The activity of MMP-9 in supernatants, and the expression of MMP-9 and CCR5 were detected at 72 hr post-infection. (A) The activity of MMP-9 was detected by zymography assay and the activity of the Ad.Null-infected cells was defined as 1. Photographs represented the gelatin zymography and histograms showed the activity of MMP-9 in Ad.PRAF3-infected and Ad.Null-infected ESCC cells. (B) The expression of MMP-9 was detected by western blot and normalized to that of β-actin. Photographs showed the western blot products and histograms showed the expression level of MMP-9 protein in Ad.PRAF3-infected and Ad.Null-infected ESCC cells. (C, D) The expression of CCR5 in total cell lysate and at plasma membrane were detected by western blot and normalized to that of β-actin. Photographs showed the western blot products and histograms showed the expression of CCR5 in total cell lysate (C) and at plasma membrane (D) in Ad.PRAF3-infected and Ad.Null-infected ESCC cells. Data represent mean ± SEM from 4 independent experiments; *, P < 0.05, **, P < 0.01 by t test.