Figure 4

Overexpression of PRAF3 inhibits the migration and invasion of ESCC cells. Eca109 and TE-1 cells were infected with Ad.PRAF3 (or Ad.Null) at a MOI of 100. Migration and invasion of cells treated with Ad.PRAF3 (or Ad.Null) were analyzed at 72 hr post-infection. (A) Wound healing assay. Photographs represented the cells migrated into the wounded area and histogram showed the relative migration distance of cells. (B) Transwell assay. Photographs represented the cells travelled through the micropore membrane and histogram showed the percentage of migrant cells. (C) Matrigel-coated transwell assay. Photographs represented the cells invaded through the matrigel and histogram showed the percentage of invasive cells. Bars = 100 μm. Data represent mean ± SEM from 4 independent experiments; **, P < 0.01 by t test.