Figure 3

Knockdown of snail and slug reverses EMT phenotype. Snail and slug knockdown resulted in an epithelial morphology (a) and reduced the doubling time of the A2780cisSN/SL cells (p-value = 0.017) compared to the A2780cis cells (b). Additionally, knockdown of snail and slug resulted in a reduction in migration from 194.0 ± 7.0 cells to 33.67 ± 3.1 cells migrating through the membrane (p-value = 0.002) as well as reducing invasion rate from 188.3 ± 4.04 cells to 56 ± 4.58 invading the matrix (p-value = 0.004), in each case, making the A2780cisSN/SL knockdown cells more similar to the A2780 cell line, having migration and invasion rates of 23.7 ± 5.1 and 36.3 ± 5.03, respectively (c). The control group, A2780cis cells transfected with a scrambled siRNA sequence, show no statistically significant changes in morphology, doubling time, migration or invasion.