Figure 3

Effect of Rapamycin, NVP-BEZ235 and PP242 on the growth of LS174T and SW480 tumor xenografts. A: An equal amount of LS174T (left panel) or SW480 (right panel) cells were harvested and injected subcutaneously into nude mice. Once the tumor reached 25 mm³ mice were randomized into four groups and treatments were started with vehicle (Control), rapamycin (R, 1.5 mg/kg/day), NVP-BEZ235 (N, 30 mg/kg/day) or PP242 (P, 60 mg/kg/day). Five mice were included in each group. Tumor volumes were evaluated using caliper measurements and calculated with the formula V = π/6 × a ² × b where a is the short axis and b the long axis of the tumor. Points, mean value of tumor volume; bars, SD. B: After 20 days of treatment, mice were sacrificed, tumor xenografts were harvested and tumor weight was measured. Columns, mean tumor weight (five tumor xenografts in each group); bars, SD. *, P < 0.05, compared to control. C: Tumor xenografts were lysed and analyzed by Western blot for phospho-S6 ribosomal protein, total S6 ribosomal protein, phospho-Akt and total Akt. The illustrated blots are representative of three independent experiments.