Isolation and genomic analysis of circulating tumor cells from castration resistant metastatic prostate cancer

BMC Cancer

Table 2 Correlation analysis of genomic profiles of spiked cells isolated from blood via IE/FACS.

Cell Line	Source	Input for WGA	No. of replicates	Correlation with positive control	S.D.
LNCaP	IE/FACS	20 cells	2	0.63	0.15
	Cell culture	50 ng	1	0.82	n.a.
PC3	IE/FACS	20 cells	2	0.88	0.03
	Cell culture	50 ng	1	0.97	n.a.
VCaP	IE/FACS	20 cells	2	0.82	0.02
	Cell culture	50 ng	1	0.89	n.a.

Isolated 20 tumor cells (in duplicate) and genomic DNA (50 ng) from cell culture were subjected to WGA and aCGH analysis and compared to positive control: unamplified genomic DNA (600 ng) from cell culture

ISSN: 1471-2407