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Table 2 A summary of confirmatory qRT-PCR analysis of selective genes

From: REGγ is associated with multiple oncogenic pathways in human cancers

Tissue

Gene Symbol

PCR value

p-value

Status

Gene Annotation

Colon

BTG2

1.25

1.5E-02

Consistent

A member of the BTG/Tob family

Lung

DAPK2

1.35

6.0E-12

Consistent

Death-associated protein kinase 1 (DAPK1)

Lung

GADD45B

1.63

2.6E-02

Consistent

Growth arrest and DNA-damage-inducible

Lung

SATB1

2.82

4.3E-04

Consistent

SATB homeobox 1

Thyroid

ABCA1

1.68

2.8E-02

Consistent

ATP-binding cassette, sub-family A

Thyroid

B3GALT4

1.32

2.0E-03

Consistent

UDP-Gal:betaGlcNAc beta 1,3-

galactosyltransferase

Thyroid

PTEN

1.29

4.3E-02

Consistent

Phosphatase and tensin homolog, tumor

suppressor

Colon

CCT3

0.62

2.9E-03

Consistent

Member of the chaperonin

Colon

DKC1

0.60

8.3E-04

Consistent

Dyskeratosis congenita 1, dyskerin

Colon

HSP90AB1

0.82

2.4E-02

Consistent

A member of heat shock

Colon

MYC

0.57

6.7E-03

Consistent

myelocytomatosis viral oncogene homolog

Colon

ODC1

0.80

1.3E-02

Consistent

A p53 target negatively regulated.

Colon

RRM2

0.66

4.0E-02

Consistent

ribonucleotide reductase M2

Liver

DDB1

0.63

3.6E-02

Consistent

Damage-specific DNA binding protein 1

Liver

HN1

0.65

1.3E-03

Consistent

Hematological and neurological expressed1

Liver

ILF2

0.79

5.7E-04

Consistent

Interleukin enhancer binding factor 2

Liver

RAN

0.75

1.3E-02

Consistent

ras-related nuclear protein

Lung

BUB3

0.82

8.2E-03

Consistent

Budding uninhibited by benzimidazoles 3 homolog

Lung

USP14

0.74

2.7E-03

Consistent

Ubiquitin specific peptidase 14

Thyroid

ATR

0.47

5.6E-03

Consistent

Ataxia telangiectasia and Rad3 related

   

Total

Consistent

N = 20 (66.7%)

Conlon

ACLY

1.40

3.7E-05

Inconsistent

ATP citrate lyase

Thyroid

TSC2

2.18

1.4E-05

Inconsistent

Tuberous sclerosis 2, tumor suppressor

Colon

CDK1

0.50

7.3E-07

Inconsistent

Cyclin-dependent kinase 1

Colon

SPPL2A

0.38

3.5E-02

Inconsistent

Signal peptide peptidase-like 2A

Conlon

TUBG1

0.43

8.5E-05

Inconsistent

Tubulin, gamma 1

Liver

EHHADH

0.61

4.4E-02

Inconsistent

Enoyl-CoA, hydratase/dehydrogenase

Liver

CYP4F2

0.70

3.2E-02

Inconsistent

Cytochrome P450, family 4, subfamily F

Lung

STARD8

0.66

7.5E-03

Inconsistent

A subfamily of Rho GTPase

Lung

UNC13A

4.73

8.2E-06

Inconsistent

Unc-13 homolog A

Thyroid

PPAP2A

0.63

2.8E-02

Inconsistent

Phosphatidic acid phosphatase type 2A

   

Total

Inconsistent

n = 10 (33.3%)

  1. PCR value was the expression level of qRT-PCR results in shR (REGγ depletion) cells relative to that in shN (REGγ expressing) cells (see Figure S4). The results were averaged from three independent experiments