Figure 5

Results of confirmatory qRT-PCR on selected genes. (A) Polar plot of qRT-PCR validation results of selected genes highly correlated with REGγ. Following qRT-PCR analysis of specific genes in REGγ knockdown or control cells, data averaged from three independent experiments were converted into relative fold changes. Fold change values greater than one was shown as positive correlation (brown) and values less than one represented negative correlation (blue). Genes were arranged in different theta (θ) and radius represent the qRT-PCR fold change values from 0 to 3. Only data consistent with prediction were shown (see Table 2). (B) Representative qPCR validation experiments. Quantitative RT-PCR was performed in paired cancer cell lines (shN and shR) with differential levels of REGγ expression. Subsets of qRT-PCR results showed the actual expression differences of a specific gene in these cell lines (results were average from three independent experiments). The relative PCR values (Table 2) in shN (usually normalized as 1) were divided by the values in shR cells to yield fold changes as shown in Figure 5A. A value greater than 1 indicates a positive correlation with REGγ.