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Table 5 Concordance between detection of MembErbB-2 expression by immunofluorescence and immunohistochemistry

From: Clinical relevance of ErbB-2/HER2 nuclear expression in breast cancer

  MembErbB-2 (IFa C-18), n (%) Total N (%) Overall
concordance (%)
κ
statisticsd
  Negative Positive    
MembErbB-2 (IHC b RBT HER2)      
   Negative 168 (83.4)c 7 (3.5) 175 (87.1) 96 0.84
   Positive 1 (0.4) 25 (12.7) 26 (12.9)   
MembErbB-2 (IHC A0485)      
   Negative 170 (80.9) 11 (5.2) 181 (86.2) 90 0.6
   Positive 10 (4.8) 19 (9.1) 29 (13.8)   
MembErbB-2 (IHC C-18)      
   Negative 168 (83.2) 9 (4.5) 177 (87.6) 93 0.7
   Positive 5 (2.5) 20 (9.9) 25 (12.4)   
  1. a IF: Immunofluorescence. b IHC: Immunohistochemistry. c Percentage of the total cohort. d κ statistics (with a value of 1.0 indicating perfect agreement and a value of -1.0 indicating perfect disagreement) revealed excellent to substantial levels of concordance between detection of MembErbB-2 positivity by IF with C-18 and by IHC with RBT-HER2, A0485, and C-18 antibodies