Opposing function of MYBBP1A expression on cell migration and proliferation in human HNSCC Cal-27. siRNA-mediated silencing of MYBBP1A expression in Cal-27 cells compared to controls (scramble siRNA) was confirmed by RQ-PCR (A) and Western blot analysis (B). Lamin B transcript levels served as control for cDNA quality and quantity, while detection of HSC70 protein levels served as control for quality and quantity of protein lysates. Significantly accelerated migration (C) and decreased proliferation (D) was observed upon silencing of MYBBP1A in Cal-27 cells. Migration was determined as described in Figure 3 and proliferation was quantified by BrdU incorporation and FACS analysis. The graph represents mean values ± SD of the percentage total of cells in S-phase. ** p-values < 0.05, *** p-values < 0.005.