Figure 4

MYBBP1A expression in human HNSCC cell lines. (A) Comparable levels of MYBBP1A transcripts were detected by semi-quantitative RT-PCR in different human tumor cell lines. LAMIN B transcript levels served as control for cDNA quality and quantity. (B) Western blot analysis with nuclear protein extracts revealed strong differences in MYBBP1A protein levels. Detection of HSC70 protein levels served as control for quality and quantity of protein lysates. (C) Increase in cell numbers of Fadu, Cal-27, SCC-4, SCC-9 and SCC-25 were counted at the indicated time points and plotted over time (*** p-values < 0.005). (D) In vitro wounding scratch assay revealed a significant higher migration rate for SCC-4, SCC-9 and SCC-25 cells compared to Fadu and Cal-27 cells. Migration was determined by measuring the closure of the gap over time and plotted as percentage total of the distance (*** p-values < 0.005).