Figure 3

Function of Mybbp1a on cell proliferation and migration in murine SCC-7 cells. (A) Murine SCC-7 cells were transfected with scramble (control) or Mybbp1a-specific siRNA (+siRNA), and reduced expression of Mybbp1a protein was confirmed in nuclear fraction using a murine anti-Mybbp1a antibody. Histone H1 levels served as internal control of protein lysate quantity and quality. Silencing of Mybbp1a was accompanied by a reduction of E-Cadherin protein levels and an Actin control reveals equal whole cell lysate loading. (B) Transient Mybbp1a down-regulation led to a reduction of DNA synthesis quantified by BrdU incorporation (*** p-value = 0.0011). (C) SCC-7 cells with silenced Mybbp1a levels showed an enhanced migratory phenotype compared to scramble transfected controls (* p-value < 0.03).