Table 2 Information of PCR product size and primer location

A. The nested PCR primer set

MUC1

Forward:

-219 bp to -200 bp

375 bp

Reverse:

+156 bp to +137 bp

MUC2

Forward:

-460 bp to -441 bp

261 bp

Reverse:

-200 bp to -219 bp

MUC3A

Forward:

-478 bp to -418 bp

495 bp

Reverse:

+17 bp to -6 bp

MUC4

Forward:

-249 bp to -228 bp

239 bp

Reverse:

-30 bp to -11 bp

MUC5AC

Forward:

-3960 bp to -3941 bp

450 bp

Reverse:

-3511 bp to -3530 bp

MUC17

Forward:

-383 bp to -361 bp

586 bp

Reverse:

+203 bp to +180 bp

B. The target PCR primer set

MUC1

Forward:

-124 bp to -15 bp

206 bp

Reverse:

+42 bp to +23 bp

MUC2

Forward:

-437 bp to -418 bp

256 bp

Reverse:

-222 bp to -241 bp

MUC3A

Forward:

-321 bp to -302 bp

361 bp

Reverse:

-10 bp to -31 bp

MUC4

Forward:

-194 bp to -173 bp

156 bp

Reverse:

-59 bp to -38 bp

MUC5AC

Forward:

-3842 bp to -3823 bp

225 bp

Reverse:

-3658 bp to -3677 bp

MUC17

Forward:

-200 bp to -181 bp

312 bp

Reverse:

+72 bp to +53 bp