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Table 2 Information of PCR product size and primer location

From: The application of methylation specific electrophoresis (MSE) to DNA methylation analysis of the 5' CpG island of mucin in cancer cells

Primer name location* Product size**
A. The nested PCR primer set
MUC1 Forward: -219 bp to -200 bp 375 bp
  Reverse: +156 bp to +137 bp  
MUC2 Forward: -460 bp to -441 bp 261 bp
  Reverse: -200 bp to -219 bp  
MUC3A Forward: -478 bp to -418 bp 495 bp
  Reverse: +17 bp to -6 bp  
MUC4 Forward: -249 bp to -228 bp 239 bp
  Reverse: -30 bp to -11 bp  
MUC5AC Forward: -3960 bp to -3941 bp 450 bp
  Reverse: -3511 bp to -3530 bp  
MUC17 Forward: -383 bp to -361 bp 586 bp
  Reverse: +203 bp to +180 bp  
B. The target PCR primer set
MUC1 Forward: -124 bp to -15 bp 206 bp
  Reverse: +42 bp to +23 bp  
MUC2 Forward: -437 bp to -418 bp 256 bp
  Reverse: -222 bp to -241 bp  
MUC3A Forward: -321 bp to -302 bp 361 bp
  Reverse: -10 bp to -31 bp  
MUC4 Forward: -194 bp to -173 bp 156 bp
  Reverse: -59 bp to -38 bp  
MUC5AC Forward: -3842 bp to -3823 bp 225 bp
  Reverse: -3658 bp to -3677 bp  
MUC17 Forward: -200 bp to -181 bp 312 bp
  Reverse: +72 bp to +53 bp  
  1. * location*: relative to transcription start site. Product size
  2. **: GC clamp added size