Primer name | sequence | Tm | |
---|---|---|---|
A. The nested PCR primer set | |||
MUC1 | Forward: | 5'-AAAGGGGGAGGTTAGTTGGA-3' | 63 |
 | Reverse: | 5'-TACCCCTCACCTATAAACAC-3' |  |
MUC2 | Forward: | 5'-TTTGGGGTTAGGTTTGGAAG-3' | 59 |
 | Reverse: | 5'-ACCTTCTTCAAAATAAAACAACC-3' |  |
MUC3A | Forward: | 5'-TTGAGGGATAGAAGGGGTTTG-3' | 64 |
 | Reverse: | 5'-AACCCCAACAACTACATAAACCC-3' |  |
MUC4 | Forward: | 5'-AGAGTAAGGGGTGTATGGGTG-3' | 60 |
 | Reverse: | 5'-ACTCCACTACCCAACAACTAC-3' |  |
MUC5AC | Forward: | 5'-AAAGTTTTGGGTGTGTGGAG-3' | 62 |
 | Reverse: | 5'-ATCAATATCCAACCCCCAAC-3' |  |
MUC17 | Forward: | 5'-ATAAAGGGGGTGTTTTTGTTAGG-3' | 62 |
 | Reverse: | 5'-AAACAAACAAAACAAACTAACCCC-3' |  |
B. The target PCR primer set | |||
MUC1 | Forward: | 5'-[GC CLAMP]AAGAGGTAGGAGGTAGGGGA-3' | 53 |
 | Reverse: | 5'-AAAACAAAACAAATTCAAAC-3' |  |
MUC2 | Forward: | 5'-[GC CLAMP]TTTTAGAGTTTGGGTTTTAG-3' | 51 |
 | Reverse: | 5'-TAACCTAAATACCAACACAC-3' |  |
MUC3A | Forward: | 5'-[GC CLAMP]TTTTAGGTAGTTTTATGTGG-3' | 52 |
 | Reverse: | 5'-AACAAAAAACTAAAACAAAAC-3' |  |
MUC4 | Forward: | 5'-[GC CLAMP] AGGAGAGAAAAGGGTGATTAG -3' | 57 |
 | Reverse: | 5'-ACTCCACTACCCAACAACTAC-3' |  |
MUC5AC | Forward: | 5'-[GC CLAMP]TTTATGTTTAGGGGTTTTGG-3' | 62 |
 | Reverse: | 5'-ACCAACTAACCACCCAAACC-3' |  |
MUC17 | Forward: | 5'-[GC CLAMP]ATTTTTATGTTTATGGGTTG-3' | 53 |
 | Reverse: | 5'-ATAATCCCTAACCTTAACATC-3' |  |