Figure 2

(A) Analysis of the methylation status of A427 (A) and LS174T (L) by MSE and conventional bisulfite-DGGE. (B) Determination of the resolution of MSE detection. A mixture of a high methylation cell line (Panc1) and a low methylation cell line (BxPC3) was analyzed. The number shows the BxPC3 contamination ratio. The upper band (arrow) shows the digitalized emission intensity using Image J. On the graph, the Y-axis shows the intensity of the upper band and the X-axis shows the BxPC3 contamination ratio. Regression equation: $\mathsf{\text{Y}}=\mathsf{\text{243}}.\mathsf{\text{26X}}+\mathsf{\text{187}}.\mathsf{\text{25}}$; correlation co-efficient: R² = 0.993. Data were averaged from 3 separate experiments.